The 218th prohibition is that we are forbidden from using two species of animals to work together.
The source of this commandment is G‑d's statement,1 "Do not plow with an ox and a donkey together."
One receives lashes for using them for any kind of work, such as plowing, threshing, or driving.2 This is because the verse says, "together," meaning that one cannot bring them together for any kind of work.
One only receives lashes by Torah law when one species is kosher and the other one non-kosher, such as "an ox and a donkey." Then, one is lashed for plowing, pulling or driving with them. One receives lashes by Rabbinic decree for using any two species [even if they are both kosher or both non-kosher].
The details of this mitzvah are explained in the eighth chapter of tractate Kilayim.
