The 86th prohibition is that we are forbidden from removing the poles [used to carry] the ark from its rings.
The source of this mitzvah is G‑d's statement1 (exalted be He), "The poles must remain in the ark's rings and they shall not be removed."
One who transgresses this prohibition is punished with lashes.
When discussing those prohibitions that are punishable by lashes in the end of tractate Makkos,2 our Sages asked, "But the Tanna omitted one who removed the poles from the ark" — i.e., that he is also punished by lashes — "which is prohibited from the verse, 'they shall not be removed.' " This shows that it counts as a prohibition and that it is punishable by lashes.