The 216th mitzvah is that we are commanded that a man must marry his brother's wife, if [that brother] died without leaving children.1

The source of this commandment is G‑d's statement2 (exalted be He) "Her yavam [i.e., the surviving brother] must cohabit with her" [i.e., the sister-in-law].

The details of this mitzvah are found in the tractate devoted to this subject, tractate Yevamos.